This is a summary of the curriculum vitae (CV) of Dr. Thomas Hoenen (PD Dr. rer. nat. habil.), Laboratory Head, Laboratory for Integrative Cell and Infection Biology, Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Greifswald, Germany.
Dr. Hoenen is a virologist who has been studying two of the most dangerous viruses, SARS and—especially—Ebola virus. He is working at the Friedrich-Loeffler-Institut in Germany, the oldest and one of the largest research institutes specialized in viruses in the world (see [link]), where he is Head of a BSL4 laboratory and Deputy Head of the BSL4 facility. BSL4 stands for biosafety level 4, which is the highest level of biosafety and comes with extraordinary responsibility.
Dr. Hoenen got his Ph.D. in virology in 2007 with summa cum laude. Despite being relatively young, he already has 92 publications, including first- or last-author papers in Science, Nature Communications, and Genome Medicine. According to Google Scholar, his publications have been cited >4600 times with an h-index of 37. His Web of Science page is [link].
He describes himself as: “I am a research scientist and for the last eight years have been heading a research group interested in understanding the virus-host interactions of highly pathogenic viruses, with a special focus on filoviruses [e.g., Ebola virus] and arenaviruses. My work aims to develop novel broadly specific therapeutic approaches against these viruses, and also to identify pathogenic determinants, and thereby allow risk assessments for newly emerging viruses. I am particularly passionate about working with young scientists, and sharing my excitement for virology and laboratory research with them [he has been supervising 23 BSc, MSc, and PhD theses].”
In 2015, he and other members of the NIH/CDC Ebola Diagnostics Team in Monrovia, Liberia, were awarded with the National Institutes of Health Director’s award for exceptional dedication in providing overseas support of Ebola diagnostic efforts in West Africa.
In 2022, he visited Nagasaki to be a keynote speaker at the 69th Annual Meeting of the Japanese Society for Virology, with a presentation titled “Studying virus-host cell interactions of Ebola virus inside and outside of BSL4 laboratories.”
It is a privilege honor that such a renown virologist, working with the most deadly viruses, will give a presentation to Fujita Health University.
CURRICULUM VITAE
EDUCATION
1998 – 2003 Diploma (Master’s degree) in Human Biology Philipps University Marburg, Germany
1999 Research trainee, Institute for Botany and Molecular Biology, University of Aachen, Germany
Activities: molecular cloning, antibody engineering
2003 Research trainee, Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Canada
Activities: cloning and expression of the SARS coronavirus S protein
2004 PhD student, Institute for Virology, Philipps University Marburg, Germany
Activities: studies regarding functions of the ebolavirus VP40 and VP24 proteins in the virus life cycle using virology, molecular biology, and cell biology techniques and reverse genetics systems
2004 – 2006 Visiting PhD student, Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Canada
Activities: studies regarding the functions of ebolavirus VP40 and VP24 in the virus life cycle using virology, molecular biology and cell biology techniques and reverse genetics systems
2004 – 2006 PhD in Virology Institute for Virology, Philipps University Marburg, Germany
Degree conferred on January 5th 2007 with summa cum laude
2021 Habilitation in Virology and appointment as Privatdozent (comparable to associate professor) University of Greifswald, Germany
POSITIONS
2007 – 2008 Postdoctoral fellow, Special Pathogens Program,National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Canada
Research: Development and application of reverse genetics systems
2008 – 2011 Postdoctoral fellow, Institute for Virology, Philipps University Marburg, Germany
Research: The lifecycle of filoviruses and arenaviruses
2011 – 2015 Visiting fellow, Laboratory of Virology, Rocky Mountain Laboratories, National Institutes of Health, Hamilton, MT, USA
Research: Highly pathogenic hemorrhagic fever viruses
2015 – 2021 Laboratory head, Laboratory for Molecular Biology of Filoviruses, Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Greifswald, Germany
Research: The molecular biology of filoviruses
2019 – present Head, BSL4 laboratory and Deputy head, BSL4 facility, Friedrich-Loeffler-Institut, Greifswald, Germany
Activities: Management of the day-to-day operations of the BSL4 laboratory/facility
2021 – present Laboratory head, Laboratory for Integrative Cell and Infection Biology, Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Greifswald, Germany
Research: Virus-host interactions of highly pathogenic viruses (in particular filo- and arenaviruses)
AWARDS
| 2015 National Institutes of Health Director’s award Awarded for exceptional dedication in providing overseas support of Ebola diagnostic efforts in West Africa as a member of the NIH/CDC Ebola Diagnostics Team in Monrovia, Liberia |
REVIEWING AND EDITING
since 2011 Ad hoc reviewer for Lancet Infectious Diseases, Science Translational Medicine, Nature Communications, Nucleic Acids Research, Emerging Microbes and Infection, Genome Medicine, Emerging Infectious Diseases, EMBO Journal, Research, Cell Reports, eLife, Antiviral Research, PLoS Pathogens, Journal of Infectious Diseases, mBio, Cells, Journal of Molecular Biology, Vaccine, Journal of Virology, Antimicrobial Agents and Chemotherapy, mSphere, Virology Journal, Viruses, Scientific Reports, RNA, RNA Biology, Journal of General Virology, PLoS Neglected Tropical Diseases, PLoS One, BMC Biotechnology, Archives of Virology
11/2014 Guest editor for PLoS Computational Biology
01/2015 Grant reviewer for the Wellcome Trust
since 2017 Member of the Review Board for Bioprotocols
since 2019 Member of the Editorial Board of Emerging Microbes & Infections
08/2020 Grant reviewer for the Biotechnology and Biological Sciences Research Council (BBRSC)
2020 Guest editor for Cells special issue “Virus – Host Cell Interactions”
since 2020 Grant reviewer for the German Research Foundation (DFG)
09/2020 Reviewer for the University of Würzburg (W1 tenure evaluation)
06/2021 Grant reviewer for the National Institutes of Health (NIH; P01 program)
since 2022 Academic editor for PLoS Pathogens
Primary publications (peer-reviewed)
(Selected publications are highlighted in bold.)
| Hu D, Wendt W, Bunz M, Kammerloher J, Kutter J, Widerspick L, Hoenen T, Schindler M. CD81 is a potent Ebola virus restriction factor and a target for host-directed antiviral therapy. Nature Microbiol (submitted NMICROBIOL-23092579). | ||
| 1. | 2023 | Bodmer BS, Vallbracht M, Ushakov DS, Wendt L, Chlanda P, Hoenen T#. Ebola virus inclusion bodies are liquid organelles whose formation is facilitated by nucleoprotein oligomerization. Emerg Microbes Infect 12(2):2223727. First study demonstrating that Ebola virus inclusion bodies are liquid organelles. |
| 2. | 2023 | Wendt L, Pickin MJ, Bodmer BS, Reiche S, Fénéant L, Hölper JE, Fuchs W, Groseth A, Hoenen T#. N6-methyladenosine is required for efficient RNA synthesis of Ebola virus and other haemorrhagic fever viruses. Emerg Microbes Infect 12(2):2223732. First study demonstrating a role of RNA methylation for the life cycle of Ebola virus. |
| 3. | 2023 | O’Donnell KL, Callison J, Feldmann H, Hoenen T, Marzi A. Single-dose treatment with VSV-EBOV expressing Ebola virus-specific artificial miRNAs does not protect mice from lethal disease. J Infect Dis 27:jiad121. |
| 4. | 2023 | Hu S, Fujita-Fujiharu Y, Sugita Y, Wendt L, Muramoto Y, Nakano M, Hoenen T, Noda T. Cryoelectron microscopic structure of the nucleoprotein-RNA complex of the European filovirus, Lloviu virus. PNAS Nexus 2(4):pgad120. |
| 5. | 2023 | Winter SL, Golani G, Lolicato F, Vallbracht M, Thiyagarajah K, Ahmed SS, Lüchtenborg C, Fackler OT, Brügger B, Hoenen T, Nickel W, Schwarz US, Chlanda P. The Ebola virus VP40 matrix layer undergoes endosomal disassembly essential for membrane fusion. EMBO J 42(11):e113578. |
| 6. | 2023 | Bohn P, Waßmann I, Wendt L, Leske A, Hoenen T, Tews BA, Groseth A. A dsRNA-binding mutant reveals only a minor role of exonuclease activity in interferon antagonism by the arenavirus nucleoprotein. PLoS Pathog. 19(1):e1011049. |
| 7. | 2023 | Bodmer BS, Breithaupt A, Heung M, Brunetti JE, Henkel C, Müller-Guhl J, Rodríguez E, Wendt L, Winter SL, Vallbracht M, Müller A, Römer S, Chlanda P, Muñoz-Fontela C, Hoenen T#, Escudero-Pérez B. In vivo characterization of the novel ebolavirus Bombali virus suggests a low pathogenic potential for humans. Emerg Microbes Infect 12(1):2164216. First reverse genetics-based rescue of Bombali virus, for which no natural isolate currently exists. |
| 8. | 2022 | Wendt L, Brandt J, Ushakov DS, Bodmer BS, Pickin MJ, Groseth A, Hoenen T#. Evidence for Viral mRNA Export from Ebola Virus Inclusion Bodies by the Nuclear RNA Export Factor NXF1. J Virol 96(18):e0090022. Detailed mechanistic description of how the cellular protein NXF1 is appropriated by Ebola virus to facilitate export of viral mRNAs from inclusion bodies. |
| 9. | 2022 | Bodmer BS, Hoenen T#. Assessment of Life Cycle Modeling Systems as Prediction Tools for a Possible Attenuation of Recombinant Ebola Viruses. Viruses 14(5):1044. |
| 10. | 2022 | Galão RP, Wilson H, Schierhorn KL, Debeljak F, Bodmer BS, Goldhill D, Hoenen T, Wilson SJ, Swanson CM, Neil SJD. TRIM25 and ZAP target the Ebola virus ribonucleoprotein complex to mediate interferon-induced restriction. PLoS Pathog 18(5):e1010530. |
| 11. | 2022 | Roman-Sosa G, Leske A, Ficht X, Dau TH, Holzerland J, Hoenen T, Beer M, Kammerer R, Schirmbeck R, Rey FA, Cordo SM, Groseth A. Immunization with GP1 but Not Core-like Particles Displaying Isolated Receptor-Binding Epitopes Elicits Virus-Neutralizing Antibodies against Junín Virus. Vaccines 10(2):173. |
| 12. | 2021 | Hunt NR, Paparisto E, Labach DS, Coleman MD, Di Gravio EJ, Dodge MJ, Li MMH, MacDonald MR, Côté M, Müller A, Hoenen T, Barr SD. Interferon-induced HERC5 inhibits Ebola virus particle production and is antagonized by Ebola glycoprotein. Cells 10(9):2399. |
| 13. | 2021 | Bhatia B, Furuyama W, Hoenen T, Feldmann H, Marzi A. Ebola Virus Glycoprotein Domains Associated with Protective Efficacy. Vaccines 9(6):630. |
| 14. | 2021 | Bodmer BS, Zierke L, Greßler J, Groseth A, Hoenen T#. Remdesivir inhibits the polymerases of the novel filoviruses Lloviu and Bombali virus. Antiviral Res 192:105120. |
| 15. | 2021 | Imamura K, Sakurai Y, Enami T, Shibukawa R, Nishi Y, Ohta A, Shu T, Kawaguchi J, Okada S, Hoenen T, Yasuda J, Inoue H. iPSC screening for drug repurposing identifies anti-RNA virus agents modulating host cell susceptibility. FEBS Open Bio 11(5):1452. |
| 16. | 2020 | Bodmer BS, Greßler J, Schmidt ML, Holzerland J, Brandt J, Braun S, Groseth A, Hoenen T#. Differences in viral RNA synthesis but not budding or entry contribute to the in vitro attenuation of Reston virus compared to Ebola virus. Microorganisms 8(8):1215. Study highlighting the ability to use reverse genetics-based modelling systems to dissect the ebolavirus life cycle into its individual aspects, and identifying the basis for the in vitro attenuation of Reston virus. |
| 17. | 2020 | Miyake T, Farley CM, Neubauer BE, Beddow TP, Hoenen T, Engel DA. Ebola virus inclusion body formation and RNA synthesis are controlled by a novel domain of NP interacting with VP35. J Virol 94(16):e02100-19. |
| 18. | 2020 | Brandt J, Wendt L, Bodmer BS, Mettenleiter TC, Hoenen T#. The cellular protein CAD is recruited into Ebola virus inclusion bodies by the nucleoprotein NP to facilitate genome replication and transcription. Cells 9(5):1126. |
| 19. | 2020 | Yoon YS, Jang Y, Hoenen T, Shin H, Lee Y, Kim M. Antiviral activity of sertindole, raloxifene and ibutamoren against transcription and replication-competent Ebola virus-like particles. BMB Rep 53(3):166-171. |
| 20. | 2020 | Wendt L, Brandt J, Bodmer BS, Reiche S, Schmidt ML, Traeger S, Hoenen T#. The Ebola virus nucleoprotein recruits the nuclear RNA export factor NXF1 into inclusion bodies to facilitate viral protein expression. Cells 9(1):187. |
| 21. | 2019 | Müller A, Fischer K, Suluku R, Hoenen T#. Sequencing of mRNA from whole blood using nanopore sequencing. J Vis Exp 148. |
| 22. | 2019 | González-Hernández M, Müller A, Hoenen T, Hoffmann M, Pöhlmann S. Calu-3cells are largely resistant to entry driven by filovirus glycoproteins and the entry defect can be rescued by directed expression of DC-SIGN or cathepsin L. Virology 532:22-29. |
| 23. | 2019 | Kämper L, Zierke L, Schmidt ML, Müller A, Wendt L, Brandt J, Hartmann E, Braun S, Holzerland J, Groseth A, Hoenen T#. Assessment of the function and intergenus-compatibility of Ebola and Lloviu virus proteins. J Gen Virol 100(5):760-772. |
| 24. | 2019 | Leske A, Waßmann I, Schnepel K, Shifflett K, Holzerland J, Bostedt L, Bohn P, Mettenleiter TC, Briggiler AM, Brignone J, Enria D, Cordo SM, Hoenen T, Groseth A. Assessing cross-reactivity of Junín virus-directed neutralizing antibodies. Antiviral Res 163:106-116. |
| 25. | 2019 | Hoffmann M, Nehlmeier I, Brinkmann C, Krähling V, Behner L, Moldenhauer AS, Krüger N, Nehls J, Schindler M, Hoenen T, Maisner A, Becker S, Pöhlmann S. Tetherin Inhibits Nipah Virus but Not Ebola Virus Replication in Fruit Bat Cells. J Virol 93(3). pii: e01821-18. |
| 26. | 2018 | Lee N, Shum D, König A, Kim H, Heo J, Min S, Lee J, Ko Y, Choi I, Lee H, Radu C, Hoenen T, Min JY, Windisch MP. High-throughput drug screening using the Ebola virus transcription- and replication-competent virus-like particle system. Antiviral Res 158:226-237. |
| 27. | 2018 | Martin S, Chiramel AI, Schmidt ML, Chen YC, Whitt N, Watt A, Dunham EC, Shifflett K, Traeger S, Leske A, Buehler E, Martellaro C, Brandt J, Wendt L, Müller A, Peitsch S, Best SM, Stech J, Finke S, Römer-Oberdörfer A, Groseth A, Feldmann H, Hoenen T#. A genome-wide siRNA screen identifies a druggable host pathway essential for the Ebola virus life cycle. Genome Med 10(1):58. Genome-wide siRNA screen of host factors important for viral RNA synthesis of Ebola virus, highlighting the power of reverse genetics-based modelling systems for high-throughput screening applications. |
| 28. | 2018 | Schmidt ML, Tews BA, Groseth A, Hoenen T#. Generation and Optimization of a Green Fluorescent Protein-Expressing Transcription and Replication-Competent Virus-Like Particle System for Ebola Virus. J Infect Dis 218(suppl_5):S360-S364. |
| 29. | 2018 | Dunham EC, Leske A, Shifflett K, Watt A, Feldmann H, Hoenen T, Groseth A. Lifecycle modelling systems support inosine monophosphate dehydrogenase (IMPDH) as a pro-viral factor and antiviral target for New World arenaviruses. Antiviral Res 157:140-150. |
| 30. | 2018 | Fischer K, Jabaty J, Suluku R, Strecker T, Groseth A, Fehling SK, Balkema-Buschmann A, Koroma B, Schmidt KM, Atherstone C, Weingartl HM, Mettenleiter TC, Groschup MH, Hoenen T, Diederich S. Serological Evidence for the Circulation of Ebolaviruses in Pigs From Sierra Leone. J Infect Dis 218(suppl_5):S305-S311. |
| 31. | 2018 | Wendt L, Kämper L, Schmidt ML, Mettenleiter TC, Hoenen T#. Analysis of a Putative Late Domain Using an Ebola Virus Transcription and Replication-Competent Virus-Like Particle System. J Infect Dis 218(suppl_5):S355-S359. |
| 32. | 2017 | Schmidt ML, Hoenen T#. Characterization of the catalytic center of the Ebola virus L polymerase. PLoS Negl Trop Dis 11(10):e0005996. |
| 33. | 2017 | Banadyga L, Hoenen T, Ambroggio X, Dunham E, Groseth A, Ebihara H. Ebola virus VP24 interacts with NP to facilitate nucleocapsid assembly and genome packaging. Sci Rep 7(1):7698. |
| 34. | 2017 | Daaboul GG, Freedman DS, Scherr SM, Carter E, Rosca A, Bernstein D, Mire CE, Agans KN, Hoenen T, Geisbert TW, Ünlü MS, Connor JH. Enhanced light microscopy visualization of virus particles from Zika virus to filamentous ebolaviruses. PLoS One 12(6):e0179728. |
| 35. | 2017 | Fuchs J, Hölzer M, Schilling M, Patzina C, Schoen A, Hoenen T, Zimmer G, Marz M, Weber F, Müller MA, Kochs G. Evolution and Antiviral Specificities of Interferon-Induced Mx Proteins of Bats against Ebola, Influenza, and Other RNA Viruses. J Virol 91(15). |
| 36. | 2017 | Nelson E, Dyall J, Hoenen T, Barnes A, Zhou H, Liang J, Michelotti J, Dewey W, Evans-DeWald L, Bennett R, Morris P, Guha R, Klumpp-Thomas C, McKnight C, Chen Y, Xu X, Wang A, Hughes E, Martin S, Thomas C, Jahrling P, Hensley L, Olinger G, White J. The Phosphatidylinositol-3-phosphate 5-kinase Inhibitor Apilimod Blocks Filoviral Entry and Infection. PLoS Negl Trop Dis 11(4):e0005540. |
| 37. | 2017 | Biava M, Caglioti C, Bordi L, Castilletti C, Colavita F, Quartu S, Nicastri E, Lauria FN, Petrosillo N, Lanini S, Hoenen T, Feldmann H, Kobinger G, Zumla A, Di Caro A, Ippolito G, Capobianchi MR, Lalle E. Detection of viral RNA in tissues following plasma clearance from an Ebola virus infected patient. PLoS Pathogens 13(1):e1006065. |
| 38. | 2016 | Hoenen T, Groseth A, Safronetz D, Wollenberg K, Feldmann H. Response to Comment on “Mutation rate and genotype variation of Ebola virus from Mali case sequences”. Science 353(6300):658. |
| 39. | 2016 | Nelson EA, Barnes AB, Wiehle RD, Fontenot GK, Hoenen T, White JM. Clomiphene and Its Isomers Block Ebola Virus Particle Entry and Infection with Similar Potency: Potential Therapeutic Implications. Viruses 8(8):206. |
| 40. | 2016 | Rosenke K, Adjemian J, Munster VJ, Marzi A, Falzarano D, Onyango CO, Ochieng M, Juma B, Fischer RJ, Prescott JB, Safronetz D, Omballa V, Owuor C, Hoenen T, Groseth A, Martellaro C, van Doremalen N, Zemtsova G, Self J, Bushmaker T, McNally K, Rowe T, Emery SL, Feldmann F, Williamson BN, Best SM, Nyenswah TG, Grolla A, Strong JE, Kobinger G, Bolay FK, Zoon KC, Stassijns J, Giuliani R, de Smet M, Nichol ST, Fields B, Sprecher A, Massaquoi M, Feldmann H, de Wit E. Plasmodium Parasitemia Associated With Increased Survival in Ebola Virus-Infected Patients. Clin Infect Dis 63(8):1026-33. |
| 41. | 2016 | de Wit E, Kramer S, Prescott J, Rosenke K, Falzarano D, Marzi A, Fischer RJ, Safronetz D, Hoenen T, Groseth A, van Doremalen N, Bushmaker T, McNally KL, Feldmann F, Williamson BN, Best SM, Ebihara H, Damiani IA, Adamson B, Zoon KC, Nyenswah TG, Bolay FK, Massaquoi M, Sprecher A, Feldmann H, Munster VJ. Clinical Chemistry of Patients with Ebola in Monrovia, Liberia. J Infect Dis 214:S303-7. |
| 42. | 2016 | de Wit E, Rosenke K, Fischer RJ, Marzi A, Prescott J, Bushmaker T, van Doremalen N, Emery SL, Falzarano D, Feldmann F, Groseth A, Hoenen T, Juma B, McNally KL, Ochieng M, Omballa V, Onyango CO, Owuor C, Rowe T, Safronetz D, Self J, Williamson BN, Zemtsova G, Grolla A, Kobinger G, Rayfield M, Ströher U, Strong JE, Best SM, Ebihara H, Zoon KC, Nichol ST, Nyenswah TG, Bolay FK, Massaquoi M, Feldmann H, Fields B. Ebola Laboratory Response at the Eternal Love Winning Africa Campus, Monrovia, Liberia, 2014-2015. J Infect Dis 214:S169-76. |
| 43. | 2016 | de Wit E, Falzarano D, Onyango C, Rosenke K, Marzi A, Ochieng M, Juma B, Fischer RJ, Prescott JB, Safronetz D, Omballa V, Owuor C, Hoenen T, Groseth A, van Doremalen N, Zemtsova G, Self J, Bushmaker T, McNally K, Rowe T, Emery SL, Feldmann F, Williamson B, Nyenswah TG, Grolla A, Strong JE, Kobinger G, Stroeher U, Rayfield M, Bolay FK, Zoon KC, Stassijns J, Tampellini L, de Smet M, Nichol ST, Fields B, Sprecher A, Feldmann H, Massaquoi M, Munster VJ. The merits of malaria diagnostics during an Ebola outbreak. Emerg Inf Dis 22(2):323-6. |
| 44. | 2016 | Hoenen T#, Groseth A, Rosenke K, Fischer RJ, Hoenen A, Judson SD, Martellaro C, Falzarano D, Marzi A, Squires RB, Wollenberg KR, de Wit E, Prescott J, Safronetz D, van Doremalen N, Bushmaker T, Feldmann F, McNally K, Bolay FK, Fields B, Sealy T, Rayfield M, Nichol ST, Zoon KC, Massaquoi M, Munster VJ, Feldmann H. Nanopore sequencing as a rapidly deployable Ebola outbreak response tool. Emerg Inf Dis 22(2):331-4. Highly cited (>220 citations on google scholar) study demonstrating the feasibility of nanopore sequencing under outbreak conditions. |
| 45. | 2016 | McCarthy SD, Majchrzak-Kita B, Racine T, Kozlowski HN, Baker DP, Hoenen T, Kobinger GP, Fish EN, Branch DR. A rapid screening assay identifies monotherapy with interferon-ß and combination therapies with nucleoside analogs as effective inhibitors of Ebola virus. PLoS NTD 10(1):e0004364. |
| 46. | 2016 | Hoenen T#. Sequencing of Ebola Virus Genomes Using Nanopore Technology. bio-protocol 6(21):e1998. |
| 47. | 2015 | Dunham EC, Banadyga L, Groseth A, Chiramel AI, Best SM, Ebihara H, Feldmann H, Hoenen T#. Assessing the contribution of interferon antagonism to the virulence of West African Ebola viruses. Nature Comm 6:8000. |
| 48. | 2015 | Gabriel G, Feldmann F, Reimer R, Thiele S, Fischer M, Hartmann E, Bader M, Ebihara H, Hoenen T, Feldmann H. Importin-α7 is required for the formation of Ebola virus inclusion bodies but is not essential for pathogenicity in mice. J Inf Dis 212 (Suppl 2):S316-21. |
| 49. | 2015 | Hoenen T#, Marzi A, Scott DP, Feldmann F, Callison J, Safronetz D, Ebihara H, Feldmann H. sGP is not required for Ebola virus virulence in guinea pigs. J Inf Dis 212 (Suppl 2):S242-6. |
| 50. | 2015 | Hoenen T, Safronetz D, Groseth A, Wollenberg KR, Koita OA, Diarra B, Fall IS, Haidara FC, Diallo F, Sanogo M, Sarro YS, Kone A, Togo AC, Traore A, Kodio M, Dosseh A, Rosenke K, de Wit E, Feldmann F, Ebihara H, Munster VJ, Zoon KC, Feldmann H, Sow S. Mutation rate and genotype variation of Ebola virus from Mali case sequences. Science 348:117-9. Study refuting earlier claims that Ebola virus shows accelerated evolution during the 2013-2016 epidemic. |
| 51. | 2015 | Tsuda Y, Hoenen T, Banadyga L, Weisend C, Ricklefs SM, Porcella SF, Ebihara H. An improved reverse genetics system to overcome cell-type dependent Ebola virus genome plasticity. J Inf Dis 212 (Suppl 2):S129-37. |
| 52. | 2014 | Hoenen T#, Groseth A, Feldmann F, Marzi A, Ebihara H, Kobinger G, Günther S, Feldmann H. Complete genome sequences of three Ebola virus isolates from the 2014 outbreak in West Africa. Genome Announcements 2(6):e01331-14. |
| 53. | 2014 | Brauburger K, Boehmann Y, Tsuda Y, Hoenen T, Olejnik J, Schümann M, Ebihara H, Mühlberger E. Analysis of the highly diverse gene borders in Ebola virus reveals a distinct mechanism of transcriptional regulation. J Virol 88(21):12558-71. |
| 54. | 2014 | Hoenen T#, Watt A, Mora A, Feldmann H. Modeling the lifecycle of Ebola virus under biosafety level 2 conditions with virus-like particles containing tetracistronic minigenomes. J Vis Exp. (91):e52381. |
| 55. | 2014 | Kuhn JH, Andersen KG, Bào Y, Bavari S, Becker S, Bennett RS, Bergman NH, Blinkova O, Bradfute S, Brister JR, Bukreyev A, Chandran K, Chepurnov AA, Davey RA, Dietzgen RG, Doggett NA, Dolnik O, Dye JM, Enterlein S, Fenimore PW, Formenty P, Freiberg AN, Garry RF, Garza NL, Gire SK, Gonzalez JP, Griffiths A, Happi CT, Hensley LE, Herbert AS, Hevey MC, Hoenen T, Honko AN, Ignatyev GM, Jahrling PB, Johnson JC, Johnson KM, Kindrachuk J, Klenk HD, Kobinger G, Kochel TJ, Lackemeyer MG, Lackner DF, Leroy EM, Lever MS, Mühlberger E, Netesov SV, Olinger GG, Omilabu SA, Palacios G, Panchal RG, Park DJ, Patterson JL, Paweska JT, Peters CJ, Pettitt J, Pitt L, Radoshitzky SR, Ryabchikova EI, Saphire EO, Sabeti PC, Sealfon R, Shestopalov AM, Smither SJ, Sullivan NJ, Swanepoel R, Takada A, Towner JS, van der Groen G, Volchkov VE, Volchkova VA, Wahl-Jensen V, Warren TK, Warfield KL, Weidmann M, Nichol ST. Filovirus RefSeq entries: evaluation and selection of filovirus type variants, type sequences, and names. Viruses 6(9):3663-82. |
| 56. | 2014 | Watt A, Moukambi F, Banadyga L, Groseth A, Callison J, Herwig A, Ebihara H, Feldmann H, Hoenen T#. A novel lifecycle modeling system for Ebola virus shows a genome length-dependent role of VP24 on virus infectivity. J Virol 88(18):10511-24. First description of a tetracistronic trVLP system, which is now widely used by numerous laboratories to study the ebolavirus lifecycle in its entirety under BSL1/BSL2 conditions. |
| 57. | 2014 | Kindrachuk J, Wahl-Jensen V, Safronetz D, Trost B, Hoenen T, Arsenault R, Feldmann F, Traynor D, Postnikova E, Kusalik A, Napper S, Blaney JE, Feldmann H, Jahrling PB. Ebola virus modulates transforming growth factor-β signaling and cellular markers of mesenchymal-like transition in hepatocytes. J Virol 88(17):9877-92. |
| 58. | 2014 | J Kuhn JH, Bào Y, Bavari S, Becker S, Bradfute S, Brauburger K, Rodney Brister J, Bukreyev AA, Caì Y, Chandran K, Davey RA, Dolnik O, Dye JM, Enterlein S, Gonzalez JP, Formenty P, Freiberg AN, Hensley LE, Hoenen T, Honko AN, Ignatyev GM, Jahrling PB, Johnson KM, Klenk HD, Kobinger G, Lackemeyer MG, Leroy EM, Lever MS, Mühlberger E, Netesov SV, Olinger GG, Palacios G, Patterson JL, Paweska JT, Pitt L, Radoshitzky SR, Ryabchikova EI, Saphire EO, Shestopalov AM, Smither SJ, Sullivan NJ, Swanepoel R, Takada A, Towner JS, van der Groen G, Volchkov VE, Volchkova VA, Wahl-Jensen V, Warren TK, Warfield KL, Weidmann M, Nichol ST. Virus nomenclature below the species level: A standardized nomenclature for filovirus strains and variants rescued from cDNA. Arch Virol 159(5):1229-37. |
| 59. | 2013 | Hoenen T#, Groseth A, Callison J, Takada A, Feldmann H. A novel ebolavirus expressing luciferase allows for rapid and quantitative testing of antivirals. Antiviral Res 99(3):207-213. |
| 60. | 2013 | Lennartz F, Hoenen T, Lehmann M, Groseth A, Garten W. The role of oligomerization for the biological functions of the arenavirus nucleoprotein. Arch Virol 158(9):1895-905. |
| 61. | 2013 | Biedenkopf N, Hartlieb B, Hoenen T, Becker S. Phosphorylation of Ebola virus VP30 influences the composition of the viral nucleocapsid complex: Impact on viral transcription and replication. J Biol Chem 288(16):11165-74. |
| 62. | 2013 | Mehedi M, Hoenen T, Robertson S, Ricklefs S, Dolan MA, Taylor T, Falzarano D, Ebihara H, Porcella SF, Feldmann H. Ebola virus RNA editing depends on the primary editing site sequence and an upstream secondary structure. PLoS Pathogens 9(10): e1003677. |
| 63. | 2013 | Shabman RS, Hoenen T, Groseth A, Jabado O, Binning JM, Amarasinghe GK, Feldmann H, Basler CF. An upstream open reading frame modulates Ebola virus polymerase translation and virus replication. PLoS Pathogens 9(1):e1003147. |
| 64. | 2012 | Hoenen T, Shabman RS, Groseth A, Herwig A, Weber M, Schudt G, Dolnik O, Basler CF, Becker S, Feldmann H. Inclusion bodies are a site of ebolavirus replication. J Virol 86(21):11779-88. First description of Ebola virus inclusion bodies as replication organelles. |
| 65. | 2012 | Groseth A, Marzi A, Hoenen T, Herwig A, Gardner D, Becker S, Ebihara H, Feldmann H. The Ebola virus glycoprotein contributes but is not sufficient for virulence in vivo. PloS Pathogens 8(8):e1002847. |
| 66. | 2011 | Spiegelberg L, Wahl-Jensen V, Kolesnikova L, Feldmann H, Becker S, Hoenen T. Genus-specific recruitment of filovirus ribonucleoprotein complexes into budding particles. J Gen Virol 92(Pt 12):2900-5. |
| 67. | 2011 | Aleksandrowicz P, Marzi A, Biedenkopf N, Beimforde N, Becker S, Hoenen T, Feldmann H, Schnittler HJ. Ebola virus enters host cells by macropinocytosis and clathrin-mediated endocytosis. J Infect Dis 204 Suppl 3:S957-67. |
| 68. | 2011 | Groseth A, Hoenen T, Weber M, Wolff S, Herwig A, Kaufmann A, Becker S. Tacaribe virus but not junin virus infection induces cytokine release from primary human monocytes and macrophages. PLoS Negl Trop Dis 5(5):e1137. |
| 69. | 2010 | Hoenen T, Jung S, Herwig A, Groseth A, Becker S. Both matrix proteins of Ebola virus regulate viral replication and transcription. Virology 403(1):56-66. |
| 70. | 2010 | Hoenen T, Biedenkopf N, Zielecki F, Jung S, Groseth A, Feldmann H, Becker S. Oligomerization of Ebola virus VP40 is essential for the formation of infectious virus-like particles. J Virol 84(14):7053-63. |
| 71. | 2010 | Wenigenrath J, Kolesnikova L, Hoenen T, Mittler E, Becker S. Establishment and application of an infectious virus-like particle system for Marburg Virus. J Gen Virol 91(Pt 5):1325-34. |
| 72. | 2010 | Groseth A, Wolff S, Strecker T, Hoenen T, Becker S. Efficient budding of Tacaribe virus matrix protein z requires the nucleoprotein. J Virol 84(7)3603-11. |
| 73. | 2009 | Groseth A, Charton JE, Sauerborn M, Feldmann F, Jones SM, Hoenen T, Feldmann H. The Ebola virus ribonucleoprotein complex: A novel VP30-L interaction identified. Virus Res 140(1-2):8-14. |
| 74. | 2007 | Groseth A, Hoenen T, Alimonti JB, Zielecki F, Ebihara H, Theriault S, Ströher U, Becker S, Feldmann H. In vitro evaluation of antisense RNA efficacy against Filovirus infection, by use of reverse genetics. J Inf Dis 196(Suppl 2):S382-389. |
| 75. | 2006 | Hoenen T, Groseth A, Kolesnikova L, Theriault S, Ebihara H, Hartlieb B, Bamberg S, Feldmann H, Ströher U, Becker S. Infection of naïve target cells with virus-like particles – implications for the role of VP24. J Virol 80(14):7260-7264. |
| 76. | 2005 | Hoenen T, Volchkov V, Kolesnikova L, Mittler E, Timmins J, Ottmann M, Reynard O, Becker S, Weissenhorn W. VP40 Octamers are essential for Ebola virus replication. J Virol 79(3):1898-1905. |
Reviews articles (peer-reviewed)
| Bodmer BS, Hoenen T#, Wendt L. The ebolavirus life cycle and its interactions with the host cell – a molecular perspective. Nature Microbiol (in revision, NMICROBIOL-23061580). | ||
| 1. | 2019 | Hoenen T#, Groseth A, Feldmann H#. Therapeutic strategies to target the Ebola virus life cycle. Nature Reviews Microbiol 17(10):593-606. |
| 2. | 2019 | Wendt L, Bostedt L, Hoenen T#, Groseth A#. High-throughput screening for negative-stranded hemorrhagic fever viruses using reverse genetics. Antiviral Res 170:104569. |
| 3. | 2019 | Brandt J, Wendt L, Hoenen T#. Structure and functions of the Ebola virus matrix protein VP40. Future Virology 14(1):21-30. |
| 4. | 2016 | Martin B, Hoenen T, Canard B, Decroly E. Filovirus proteins for antiviral drug discovery: A structure/function analysis of surface glycoproteins and virus entry. Antiviral Res 135:1-14. |
| 5. | 2014 | Hoenen T, Feldmann H. Ebolavirus in West Africa, and the use of experimental therapies or vaccines. BMC Biol 12(1):80. |
| 6. | 2014 | Hoenen T#, Feldmann H. Reverse genetics systems as tools for the development of novel therapies against filoviruses. Exp Rev Anti Infect Ther 12(10):1253-63. |
| 7. | 2014 | Falzarano D, Groseth A, Hoenen T#. Development and application of reporter-expressing mononegaviruses: current challenges and perspectives. Antiviral Res 103:78-87. |
| 8. | 2012 | Hoenen T, Groseth A, Feldmann H. Current Ebola vaccines. Expert Opin Biol Ther 12(7):859-72. |
| 9. | 2011 | Hoenen T, Groseth A, de-Kok-Mecrado F, Kuhn J, Wahl-Jensen V. Minigenomes, transcription and replication competent virus-like particles and beyond: reverse genetics systems for filoviruses and other negative stranded hemorrhagic fever viruses. Antiviral Res 91(2):195-208. |
| 10. | 2007 | Hoenen T, Kolesnikova L, Becker S. Filovirus- and Arenavirus-like particles. Future Virology 2(2):193-203. |
| 11. | 2006 | Hoenen T, Groseth A, Falzarano D, Feldmann H. Ebola virus: Unraveling pathogenesis to combat a deadly disease. Trends Mol Med 12(5):206-215. |
Book chapters
| 1. | 2024 | Bodmer BS, Hoenen T#. Reverse Genetics Systems for Filoviruses. In:Perez DR (Ed.), Reverse Genetics of RNA Viruses. Springer Science and Business Media LLC, New York, USA. (in press) |
| 2. | 2021 | Fénéant L, Bodmer BS, Mettenleiter TC, Groseth A, Hoenen T#. Current Therapies for Biosafety Level 4 Pathogens. In: Rübsamen-Schaeff H (Ed.), New Developments in Antiviral Drugs. Wiley VCH- Verlag, Weinheim, Germany. |
| 3. | 2018 | Hoenen T#. Minigenome Systems for Filoviruses. In: Salvato M (Ed.), Hemorrhagic Fever Viruses: Methods and Protocols. Springer Science and Business Media LLC, New York, USA. |
| 4. | 2017 | Hoenen T, Brandt J, Caì Y, Kuhn JH, Finch C. Reverse Genetics of Filoviruses. In:Mühlberger E, Hensley LL, Towner JS (Eds.), Marburg- and Ebolaviruses. Springer International Publishing AG, Cham, Switzerland. |
| 5. | 2017 | Hoenen T#. Luciferase-Expressing Ebolaviruses as Tools for Screening of Antivirals. In: Hoenen T, Groseth A (Eds.), Ebolaviruses: Methods and Protocols. Springer Science and Business Media LLC, New York, USA. |
| 6. | 2017 | Biedenkopf N, Hoenen T#. Modeling the Ebolavirus Life Cycle with Transcription and Replication-Competent Viruslike Particle Assays. In: Hoenen T, Groseth A (Eds.), Ebolaviruses: Methods and Protocols. Springer Science and Business Media LLC, New York, USA. |
| 7. | 2017 | Groseth A, Hoenen T. Forty Years of Ebolavirus Molecular Biology: Understanding a Novel Disease Agent Through the Development and Application of New Technologies. In: Hoenen T, Groseth A (Eds.), Ebolaviruses: Methods and Protocols. Springer Science and Business Media LLC, New York, USA. |
| 8. | 2017 | Hoenen T#, Feldmann H. Reverse Genetics Systems for Filoviruses. In:Perez DR (Ed.), Reverse Genetics of RNA Viruses. Springer Science and Business Media LLC, New York, USA. |
| 9. | 2015 | Groseth A, Eickmann M, Ebihara H, Becker S, Hoenen T#. Filoviruses: Ebola, Marburg and disease. In: Encyclopaedia of Life Sciences. John Wiley & Sons, Sussex, United Kingdom. |
| 10. | 2013 | Hoenen T#. Ebolavirus vaccines. In: M. Briese (Ed.), Molecular Vaccines. Springer Science and Business Media LLC, New York, USA. |
| 11. | 2011 | Groseth A, Hoenen T, Eickmann M, Becker S. Filoviruses: Ebola, Marburg and disease. In: Encyclopaedia of Life Sciences. John Wiley & Sons, Sussex, United Kingdom. |
| 12. | 2007 | Hoenen T, Becker S. [Marburg virus hemorrhagic fever](German). In: F. Hofmann (Ed.), Handbuch der Infektionskrankheiten. ecomed Verlag, Landsberg 22. Ergänzungslieferung 10/07, ecomed Verlag, Landsberg, Germany. |
| 13. | 2007 | Hoenen T, Gottschalk R, Becker S. [Ebola virus hemorrhagic fever](German). In: F. Hofmann (Ed.), Handbuch der Infektionskrankheiten 20. Ergänzungslieferung 06/07, ecomed Verlag, Landsberg, Germany. |
Books
| 1. | 2020 | Hoenen T, Groseth A. Virus – host cell interactions. Cells Special Issue. MDPI. ISBN: 978-3-0365-6559-0. |
| 2. | 2017 | Hoenen T, Groseth A. Ebolaviruses – Methods and Protocols. Methods in Molecular Biology 1628. Springer Science and Business Media LLC, New York, USA. ISBN 978-1-4939-7115-2 |
Other publications
| 1. | 2023 | Hoenen T#, Groseth A. [Reverse genetic systems for the study of Ebola viruses](German). BIOspektrum 29(2):153. |
| 2. | 2022 | Hoenen T#, Groseth A. Editorial: Virus-Host Cell Interactions. Cells 11(5):804. |
| 3. | 2019 | Fischer K, Diederich S, Hoenen T. [The role of livestock and companion animals in Ebola virus biology – The EbolaForesight Project](German). Ernährung im Fokus 2019(3):214. |
