Science Portrait: Prof. Mark O. Huising

This is a summary of the curriculum vitae (CV) of Prof. Mark O. Huising, PhD, Neurobiology, Physiology & Behavior, College of Biological Sciences and Physiology & Membrane Biology, School of Medicine, University of California, Davis, USA. On July 29, he will give a presentation at Fujita Health University. Prof. Huising is originally from the Netherlands, where he studied Biology, and since 2006 has been living in the USA where, since 2014, he leads a group at UC Davis.

Prof. Huising and his group study how different cell types in pancreatic islets communicate to control their production of insulin (by beta cells) and glucagon (by alpha cells). The levels of these hormones need to be tightly controlled for our bodies to deal with fluctuating glucose levels. In healthy islets, alpha and beta-cell output are coordinated via feedback from delta cells. In diabetes—a scourge of modern civilization—the crosstalk between pancreatic islet cells breaks down, which aggravates disease.

Prof. Huising is a leader in this field, with first and/or corresponding authorships on papers in the prestigious journals Nature Medicine, Nature Reviews in Endocrinology, Cell Metabolism (2017, 2018), PNAS, Diabetologia (2015, 2020), and Nature Metabolism. As a co-author, he also has a paper in Cell.

One of the main (aggravating) causes of diabetes is a reduction in beta-cell capacity. The paradigm used to be that, in adult pancreas, new beta-cells are exclusively generated through beta-cell self-replication.  However, Prof. Huising and his group identified a new population of immature beta-cells in an adult pancreatic islet niche and provided evidence suggesting that these cells can develop from alpha-cells and progress into mature functional beta-cells (Van der Meulen et al., Cell Metabolism 2017). This islet cell plasticity offers alternative therapeutic targets for increasing the number of beta-cells. The paper was featured in Cell Metabolism’s “Best of 2017” issue and awarded the College of Biological Sciences Faculty Research Award at UC Davis.

Before becoming professor at UC Davis, from 2006-2012 Prof. Huising worked at The Salk Institute for Biological Studies, La Jolla in the group of the late Prof. Wylie W. Vale, a giant in the field of neuroendocrinology and molecular neurobiology. In the 1970s and 1980s, Prof. Vale discovered several hormones, among which neuropeptide corticotropin-releasing factor (CRF) which is involved in stress responses. In 2010, Prof. Huising published in PNAS that CRF also plays a part in the pancreas, where it increases insulin secretion and promotes the division of the insulin-producing beta cells. Because this might provide an alternative route for overcoming beta-cell deficiencies, the article was even featured in the Wall Street Journal.

I know Prof. Huising and his long-time collaborator—his then-girlfriend and now-wife—Prof. Talitha van der Meulen, from his early career days, when as a PhD student he studied the overlap between endocrinology and immunology in fish. He sent me a copy of his 2006 PhD thesis “Communication in the Endocrine and Immune Systems: Physiology and Phylogeny,” which features 10 (!) first author papers although only five are obligatory for obtaining a Dutch PhD degree in Biology. The thesis was predominantly about the functions and phylogeny of molecule families that include hormones, cytokines, and/or chemokines. I am also a specialist in this field and found it the best PhD thesis that I had ever seen for several reasons: (1) It needed a large amount of very hard work to perform these analyses seriously; (2) It needed a lot of talent, especially at that young age, to be able to extract valuable and elegant conclusions from such a wide topic; for example, the review Huising et al. 2006 on type-1 cytokines remains fresh and probably is the best of its kind even to date; (3) A large effort was made to have everything look beautiful and insightful, yet playful; (4) This was done in a research group where at the time no one else had similar skills; (5) In the type of work, many reviewers can easily be fooled, but Prof. Huising did not take cheap shots and also diligently investigated issues that to most others are not immediately appealing, which reveals a sincere passion for science. Apparently, I was not the only one who admired his thesis, as his university (Radboud University) awarded it with the highest honors and by the Royal Dutch Zoological Society it was awarded as the best thesis in the field of Biology, 2006. The work revealed enormous willpower and ambition, and it made sense that Prof. Huising changed research species and country to where there are more opportunities.

It is because of his inner fire and genuine dedication to science that I am sure that Prof. Huising will always be a frontrunner scientist, regardless of the topic. Therefore, I am very happy and honored that he will give a presentation at Fujita Health University. You will have a great opportunity to learn more about diabetes and pancreatic islet crosstalk.

My Science

(a personal statement about his science by Professor Huising)

I started my scientific journey investigating the evolution of crosstalk in the immune and endocrine systems of early vertebrate species. This work on stress hormones landed me an invitation to join the group of the late Dr. Wylie Vale, who made major contributions to our understanding of hypothalamic control over the pituitary gland. In Wylie’s group, I started working on crosstalk between the endocrine cells types that make up pancreatic islets. In the decade since, I have developed a series of models that enable the realtime quantification of pancreatic islet cell behaviors using a suite of genetically encoded biosensors for calcium, cAMP, and more. Among my findings was the discovery of the natural break on insulin secretion that prevents inadvertent and dangerous excess insulin secretion. I love a good video to show the complex coordination of islet cell behavior.

 

CURRICULUM VITAE

 

EDUCATION:

 

1995-2000          Undergraduate student, Wageningen University, Wageningen, The Netherlands.

Degree obtained: MSc in Biology

Areas of study: Immunology and Developmental Biology

Faculty Advisors: B.M. Lidy Verburg-van Kemenade, PhD

Honors: Degree awarded with highest honors

In 2000: Undergraduate Student, Oregon Health Sciences University (OHSU), Portland, USA.

Faculty Advisor: Mary Stenzel-Poore, PhD

 

2000-2006            Radboud University Nijmegen, Nijmegen, The Netherlands

Degree obtained: PhD in Science

The work was done in collaboration with Wageningen University, The Netherlands

Areas of study: Comparative Neuroendocrinology and Immunology

Title of Thesis: Communication in the Endocrine and Immune Systems: Physiology and Phylogeny (ISBN-13: 978-90-9020265-5).

Honors: Degree awarded with highest honors and the PhD dissertation was awarded the prestigious ‘Dutch Zoology Award’ in 2006.

Faculty Advisors: Gert Flik, PhD, B.M. Lidy Verburg-van Kemenade, PhD and Huub F.J. Savelkoul, PhD

Job Responsibility/Achievements: Several novel lines of scientific research were successfully initiated, which resulted in 23 publications in international peer-reviewed scientific journals, including a paper that was awarded the Annual Graduate School Publication Prize in 2004. Guest lecturer and lab assistant for undergraduate level biology courses. Scientific advisor and mentor to six undergraduate students, five of whom have subsequently completed post-graduate degrees elsewhere.

 

2006       SMBWO (Dutch Society for Scientific Researchers in Medical Biology)

Completed practical and theoretical training as a certified Immunologist

 

PROFESSIONAL EXPERIENCE:

 

2006-2010\2010-2012  Senior Research Associate/Research Associate

The Salk Institute for Biological Studies, La Jolla

Faculty Advisor: Wylie W. Vale, PhD

Job Responsibility/Achievements: Initiator and lead investigator on two key research projects in the Peptide Biology Department that focus on diabetes and obesity. Work has resulted in publications in leading international peer-reviewed scientific journals and was recognized with the Endocrine Scholars Award in 2007 for Outstanding Achievement in Basic Endocrine Research and with the 2011 Novo Nordisk Award for endocrinology.

 

2012-2014 Staff Scientist

 The Salk Institute for Biological Studies, La Jolla

Job Responsibility/Achievements: Initiator and lead investigator on two key research projects with therapeutic potential for the treatment of type 1 and type 2 diabetes and obesity. Work was awarded in 2013 with a career development award by the Juvenile Diabetes Research to assist exceptionally promising investigators to position themselves at the leading edge of type 1 diabetes research.

 

2014-present   Professor

Department of Neurobiology, Physiology and Behavior, & Department of Physiology and Membrane Biology, University of California, Davis.

 

AWARDS:

 

2002  Socrates/Erasmus Scholarship.

2004  DAAD Fellowship (German Academic Exchange Service)

2004  Recipient of the 2004 Annual Publication Prize of the WIAS graduate school.

2006  Recipient of the Dutch Zoology Award 2006, awarded by the Royal Dutch Zoological Society for the best thesis in the field of Biology

2006  Recipient of a Goodlife Healthcare Travel Grant to visit the Annual meeting of the Endocrine Society 2007 in Toronto, Canada

2007  Recipient of the Endocrine Scholars Award 2007, awarded by the US Endocrine Society for Outstanding Achievement in Basic Endocrine Research

2008  Recipient of an Endocrine Trainee Award, class of 2008, awarded by the US Endocrine Society to the brightest graduate students, postdocs and clinical fellows in Endocrinology

2010  Keystone Symposia Scholarship to attend the Symposium on Islet Biology, Whistler, Canada

2011  Recipient of the 2011 Novo Nordisk Award for Endocrinology for important contributions to the field of human growth, diabetes mellitus, osteoporosis and medical technology assessment

2013  Recipient of a Career Development Award from the Juvenile Diabetes Research Foundation to assist exceptionally promising investigators position themselves to work at the leading edge of type 1 diabetes research

2014  Recipient of an American Diabetes Association Travel Award, for the best abstracts by early career scientists, to attend the 2014 ADA Scientific Sessions in San Francisco, CA

2015  Recipient of a 2015 Helmsley Award to recognize outstanding work on the underlying mechanisms and causes of Type 1 Diabetes, in support of attendance to the 2015 Endocrine Society Annual meeting in San Diego, CA

2015  Recipient of a Hartwell Individual Biomedical Research Award, awarded competitively to fund early stage, transformative biomedical research with the potential to benefit children

2016  Nominated for the 2016 Helmholtz Young Investigator Diabetes Award and invited to present at the 4th Annual Helmholtz Nature Medicine Diabetes Conference

2018  Invited member of the Brehm Coalition, a National think tank of leaders in Type 1 Diabetes

2018  Recipient of the Faculty Research Award of the College of Biological Sciences, UC Davis.

2019  John Hutton Lecture, for a rising star in the islet community in Western North America

2020  Elected Fellow of the American Association for the Advancement of Science (AAAS)

2021  UC Davis Chancellor’s Fellow, awarded annually to up and coming investigators

2021  UC Davis Faculty Sustainability Champion

 

REVIEWER AND EDITOR FUNCTIONS:

2012-present     Editorial board member for The Journal of Endocrinology and Journal of Molecular Endocrinology

2000-present     Career reviewer record: 216 verified reviews of 163 manuscripts for leading international peer-reviewed scientific journals including: Nature, Science, Cell Metabolism, Nature Metabolism, Current Biology, Journal of Clinical Investigation, Nature Communications, Diabetes, Scientific Reports, Diabetologia, Endocrinology, Genome Research, and others. https://publons.com/author/481089/mark-o-huising#profile

 

OTHER PROFESSIONAL SERVICES:

2019       Scientific panel member convened to advise NIDDK’s future planning for The Special Diabetes Program, appropriated specifically for Type 1 Diabetes Research at $150 million annually (T1Diabetes.nih.gov)

2019       Scientific panel member of the Islet Targeting Strategy Workshop to advise the JDRF on multi-disciplinary strategies to solve the outstanding problem of targeting drugs to islets

2018 and 2020 External evaluator of the Human Islet Research Network (HIRN) program, an NIH-administered consortium for diabetes research (3-day site visit, Washington DC, May 2018), with follow up in June 2020

2013 – present Ad hoc reviewer for NIDDK (BMDM, CADO, MCE, DP1, HIRN, special emphasis (2x)), Diabetes UK (4x), the European Research Council (2x), the Juvenile Diabetes Research Foundation (9x), AAAS (1x), the FWO (2x), the Swiss National Science Foundation (2x), the Singapore Medical Research Council (1x), and UC Mexus (1x)

2000-2003          Member of the Board of Supervisory Directors of the Social Housing Corporation Wageningen (SSHW), a rental corporation for students with approximately 4500 tenants

1996-1999          Member of the SFO, a non-profit foundation that looks after the interests of the tenants of the Social Housing Corporation Wageningen (SSHW)

 

Patents

HUISING MO, Matsumoto M, Vale WW. Methods for increasing insulin secretion by co-stimulation of corticotropin-releasing factor receptors. US Patent No. 9314506. Issue date 04/19/2016

 

PUBLICATIONS

1. ENGELSMA MY, HUISING MO, VAN MUISWINKEL WB, FLIK G, KWANG J, SAVELKOUL HF, VERBURG-VAN KEMENADE BML. 2002 Neuroendocrine-immune interactions in fish: a role for interleukin-1. Veterinary Immunology and Immunopathology 87(3-4):467-79.

2. HUISING MO, GUICHELAAR T, HOEK C, VERBURG-VAN KEMENADE BML, FLIK G, SAVELKOUL HF, ROMBOUT JHWM. 2003 Increased efficacy of immersion vaccination in fish with hyperosmotic pretreatment. Vaccine 21(27-30):4178-4193.

3. HUISING MO, STET RJ, KRUISWIJK CP, SAVELKOUL HF, VERBURG-VAN KEMENADE BML. 2003 Molecular evolution of CXC chemokines: extant CXC chemokines originate from the CNS. Trends in Immunology 24(6):307-313.

4. HUISING MO, STOLTE E, FLIK G, SAVELKOUL HF, VERBURG-VAN KEMENADE BML. 2003 CXC chemokines and leukocyte chemotaxis in common carp (Cyprinus carpio L.). Developmental and Comparative Immunology 27(10):875-888.

5. HUISING MO, VAN DER MEULEN T, FLIK G, VERBURG-VAN KEMENADE BML. 2004 Three novel carp CXC chemokines are expressed early in ontogeny and at nonimmune sites. European Journal of Biochemistry 271(20):4094-4106.

6. HUISING MO, STET RJ, SAVELKOUL HF, VERBURG-VAN KEMENADE BML. 2004 The molecular evolution of the interleukin-1 family of cytokines; IL-18 in teleost fish. Developmental and Comparative Immunology 28(5):395-413.

7. HUISING MO, METZ JR, VAN SCHOOTEN C, TAVERNE-THIELE AJ, HERMSEN T, VERBURGVAN KEMENADE BML, FLIK G. 2004 Structural characterisation of a cyprinid (Cyprinus carpio L.) CRH, CRH-BP and CRH-R1, and the role of these proteins in the acute stress response. Journal of Molecular Endocrinology 32(3):627-648.

8. MURRAY SE, ROSENZWEIG HL, JOHNSON M., HUISING MO, SAWICKI K, STENZEL-POORE MP. 2004 Overproduction of corticotropin-releasing hormone blocks germinal center formation: role of corticosterone and impaired follicular dendritic cell networks. Journal of Neuroimmunology 156(1-2):31-41.

9. METZ JR, HUISING MO, MEEK J, TAVERNE-THIELE AJ, WENDELAAR BONGA SE, FLIK G. 2004 Localization, expression and control of adrenocorticotropic hormone in the nucleus preopticus and pituitary gland of common carp (Cyprinus carpio L.). Journal of Endocrinology 182(1):23-31.

10. HUISING MO, METZ JR, DE MAZON AF, VERBURG-VAN KEMENADE BML, FLIK G. 2005 Regulation of the stress response in early vertebrates. Trends in Comparative Endocrinology and Neurobiology in: Annals of the New York Academy of Sciences 1040:345-347.

11. HUISING MO, KRUISWIJK CP, VAN SCHIJNDEL JE, SAVELKOUL HFJ, FLIK G, VERBURG-VAN KEMENADE BML. 2005 Multiple and highly divergent IL-11 genes in teleost fish. Immunogenetics 57(6):432-43.

12. HUTTENHUIS HB, HUISING MO, VAN DER MEULEN T, OOSTERHOUD CN, SANCHEZ NA, TAVERNE-THIELE AJ, STROBAND HW, ROMBOUT JH. 2005 Rag expression identifies B and T cell lymphopoietic tissues during the development of common carp (Cyprinus carpio). Developmental and Comparative Immunology 29(12):1033-47.

13. HUISING MO, FLIK G. 2005 The remarkable conservation of corticotropin-releasing hormone (CRH)-binding protein in the Honeybee (Apis mellifera) dates the CRH system to a common ancestor of insects and vertebrates. Endocrinology 146(5):2165-70.

14. VAN DER MEULEN T, SCHIPPER H, VAN DEN BOOGAART JG, HUISING MO, KRANENBARG S, VAN LEEUWEN JL. 2006 Endurance exercise differentially stimulates heart and axial muscle development in zebrafish (Danio rerio). American Journal of Physiology – Regulatory, Integrative and Comparative Physiology 291(4):R1040-R1048.

15. MAZON AF, VERBURG-VAN KEMENADE BML, FLIK G, HUISING MO. 2006 Corticotropin-releasing hormone-receptor 1 (CRH-R1) and CRH-binding protein (CRH-BP) are expressed in the gills and skin of common carp Cyprinus carpio L. and respond to acute stress and infection. The Journal of Experimental Biology 209:510-517.

16. HUISING MO, VAN SCHIJNDEL JE, KRUISWIJK CP, NABUURS SB, SAVELKOUL HFJ, FLIK G, VERBURG-VAN KEMENADE BML. 2006 The presence of multiple and differentially regulated interleukin-12p40 genes in bony fishes signifies an expansion of the vertebrate heterodimeric cytokine family. Molecular Immunology 43:1519-1533.

17. HUISING MO, GEVEN EJW, KRUISWIJK CP, NABUURS SB, STOLTE EH, SPANINGS FAT, VERBURG-VAN KEMENADE BML, FLIK G. 2006 Increased leptin expression in common carp (Cyprinus carpio) after food intake but not after fasting or feeding to satiation. Endocrinology 147(12):5786-5797. Recommended by the Faculty of 1000 Biology

18. METZ JR, HUISING MO, LEON K, VERBURG-VAN KEMENADE BML, FLIK G. 2006 Central and peripheral interleukin-1b and interleukin-1 receptor I expression and their role in the acute stress response of common carp, Cyprinus carpio L. Journal of Endocrinology 191(1):25-35.

19. FLIK G, KLAREN PHM, VAN DEN BURG E, METZ JR, HUISING MO. 2006 CRF and stress in fish. General and Comparative Endocrinology 146:36-44.

20. GORISSEN M, FLIK G, HUISING MO. 2006 Peptides and Proteins regulating food intake: a comparative view. Animal Biology 56(4):447-473.

21. HUISING MO, KRUISWIJK CP, FLIK G. 2006 Phylogeny and evolution of class-I helical cytokines. Journal of Endocrinology 189(1):1-25.

22. MAZON AF, HUISING MO, TAVERNE-THIELE AJ, BASTIAANS J, VERBURG-VAN KEMENADE BML, FLIK G. 2007 The first appearance of rodlet cells in carp (Cyprinus carpio L.) ontogeny and their possible roles during stress and parasite infection. Fish and Shellfish Immunology 22(1-2):27-37.

23. GONZALEZ SF, HUISING MO, STAKAUSKAS R, FORLENZA M, VERBURG-VAN KEMENADE BML, BUCHMANN K, NIELSEN ME, WIEGERTJES GF. 2007 Real-time gene expression analysis in carp (Cyprinus carpio L.) skin: inflammatory responses to injury mimicking infection with ectoparasites. Developmental and Comparative Immunology 31(3):244-254.

24. HUISING MO, VAN DER AA LM, METZ JR, DE FATIMA MAZON A, VERBURG-VAN KEMENADE BML, FLIK G. 2007 Corticotropin-releasing factor (CRF) and CRF-binding protein expression in and release from the head kidney of common carp: evolutionary conservation of the adrenal CRF system. Journal of Endocrinology 193(3):349-357.

25. HUISING MO, VAUGHAN JM, SHAH SH, GRILLOT KL, DONALDSON CJ, RIVIER J, FLIK G, VALE WW. 2008 Residues of corticotropin releasing factor-binding protein (CRF-BP) that selectively abrogate binding to CRF but not to urocortin 1. Journal of Biological Chemistry 283(14):8902-8912.

26. GORISSEN M, BERNIER NJ, NABUURS SB, FLIK G, HUISING MO. 2009 Two divergent leptin paralogues in zebrafish (Danio rerio) that originate early in teleostean evolution. Journal of Endocrinology 201(3):329-339.

27. HUISING MO, VAN DER MEULEN T, VAUGHAN JM, DONALDSON CJ, PARK H, BILLESTRUP N, VALE WW. 2010 Corticotropin releasing factor receptor 1 (CRFR1) is expressed on pancreatic b cells, promotes b cell proliferation and potentiates insulin secretion in an incretin-like manner. Proceedings of the National Academy of Sciences USA 107(2):912-917. Featured in the Wall Street Journal

28. GORISSEN M, DE VRIEZE E, FLIK G, HUISING MO. 2011 STAT genes display differential evolutionary rates that correlate with their roles in the endocrine and immune system. Journal of Endocrinology 209(2):175-184.

29. HUISING MO, PILBROW A, MATSUMOTO M, VAN DER MEULEN T, PARK H, DONALDSON CJ, VAUGHAN JM, LEE, SL, VALE WW. 2011 Glucocorticoids differentially regulate the expression of CRFR1 and CRFR2a in MIN6 insulinoma cells and rodent islets. Endocrinology 152(1):138-150.

30. GORISSEN M, BERNIER NJ, MANUEL R, DE GELDER S, METZ JR, HUISING MO, FLIK G. 2012 Recombinant human leptin attenuates stress axis activity in common carp (Cyprinus carpio L.). General and Comparative Endocrinology 178(1):75-81.

31. VAN DER MEULEN T, XIE R, KELLY OG, VALE WW, SANDER M, HUISING MO. 2012 Urocortin 3 marks mature human primary and embryonic stem cell-derived pancreatic alpha and beta cells. PLoS ONE 7(12): e52181.

32. JENSEN MV, HALDEMAN JM, ZHANG H, LU D, HUISING MO, VALE WW, HOHMEIER HE, ROSENBERG P, NEWGARD CB 2013 Control of Kv2.2 expression by pyruvate-isocitrate cycling regulates glucose-stimulated insulin secretion. Journal of Biological Chemistry 288(32):23128-40.

33. MANUEL R, METZ JR, FLIK G, VALE WW, HUISING MO. 2014 Corticotropin-releasing factor-binding protein (CRF-BP) inhibits CRF- and urotensin-I-mediated activation of CRF receptor-1 and -2 in common carp. General and Comparative Endocrinology 24;202C:69-75.

34. REIRA CE, HUISING MO, FOLLET P, LEBLANC M, VAN ANDEL R, DE MAGHALAES FILHO CD, MERKWIRTH C, DILLIN A. 2014 TRPV1 pain receptors regulate longevity and metabolism by neuropeptide signaling. Cell 157:1023-1036. Topic of a Preview in: Cell (Steculorum and Bruning, 157:1004-1006).

35. BENNER C, VAN DER MEULEN T, CACERES E, TIGYI K, DONALDSON CJ, HUISING MO. 2014 The transcriptional landscape of mouse beta cells compared to human beta cells reveals notable species differences in long non-coding RNA and protein-coding gene expression. BMC Genomics 15(1):620.

36. VAN DER MEULEN T, HUISING MO. 2014 Maturation of Stem Cell-Derived Beta cells Guided by the Expression of Urocortin 3. The Review of Diabetic Studies 11(1):115-132.

37. BLAABJERG L CHRISTENSEN GL, MATSUMOTO M, HUISING MO, VAN DER MEULEN T, BILLESTRUP N, VALE WW. 2014 CRFR1 activation protects against cytokine-induced beta cell death. Journal of Molecular Endocrinology 53(3):417-427.

38. PROTHIWA M, SYED I, HUISING MO, VAN DER MEULEN T, DONALDSON CJ, TRAUGER SA, KAHN BB, SAGHATELIAN A. 2014 Data-driven synthesis of proteolysis-resistant peptide hormones. Journal of the American Chemical Society 136(51):17710-17713.

39. VAN DER MEULEN T and HUISING MO. 2015 Role of transcription factors in the transdifferentiation of pancreatic islet cells. Journal of Molecular Endocrinology 54(2):R103-117.

40. HUISING MO. 2015 Tuning to the right signal. Diabetologia 58(6):1146-1148.

41. HOGAN MF, RAVNSKJAER K, MATSUMURA S, HUISING MO, HULL RL, KAHN SE, MONTMINY M. 2015 Hepatic insulin resistance following chronic activation of the CREB coactivator CRTC2, J Biol. Chem. 290(43):25997-26006.

42. VAN DER MEULEN T, DONALDSON CJ, CACERES E, HUNTER AE, COWING-ZITRON C, POUND LD, ADAMS MW, ZEMBRZYCKI A, GROVE KL, HUISING MO. 2015 Urocortin3 mediates somatostatin-dependent negative feedback control of insulin secretion, Nature Medicine 21(7):769-776. Topic of an Editors Choice in: Science Signaling (W. Wong).

43. DIGRUCCIO MR, MAWLA AM, DONALDSON CJ, NOGUCHI GM, VAUGHAN J, COWING-ZITRON C, VAN DER MEULEN T, HUISING MO. 2016 Comprehensive alpha, beta and delta cell transcriptomes reveal that ghrelin selectively activates delta cells and promotes somatostatin release from pancreatic islets. Molecular Metabolism 5:449-458. Topic of an Editorial in: Molecular Metabolism (Tong and Mauvais-Jarvis, 5:433-434).

44. PILBROW AP, LEWIS KA, PERRIN MH, SWEET WE, MORAVEC CS, TANG WH, HUISING MO, TROUGHTON RW, CAMERON VA. 2016 Cardiac CRFR1 Expression Is Elevated in Human Heart Failure and Modulated by Genetic Variation and Alternative Splicing. Endocrinology 157(12):4865-4874.

45. PAGLIALUNGA S, SIMNETT G, ROBSON H, HOANG M, PILLAI R, ARKELL AM, SIMPSON JA, BONEN A, HUISING MO, JOSEPH JW, HOLLOWAY GP. 2017 The Rab-GTPase activating protein, TBC1D1, is critical for maintaining normal glucose homeostasis and β-cell mass. Applied Physiology, Nutrition, and Metabolism 42(6):647-655

46. PULLEN TJ, HUISING MO, RUTTER GA. 2017 Analysis of purified pancreatic islet beta and alpha cell transcriptomes reveals 11β-hydroxysteroid dehydrogenase (Hsd11b1) as a novel disallowed gene. Frontiers in Genetics 8:41.

47. VILARINO M, RASHID ST, SUCHY FP, MCNABB BR, VAN DER MEULEN T, FINE EJ, AHSAN S, MURSALIYEV N, SEBASTIANO V, DIABB SS, HUISING MO, NAKAUCHI H, ROSS PJ. 2017 CRISPR/Cas9 microinjection in oocytes disables pancreas development in sheep. Scientific Reports 12:17472.

48. ZENG C, MULAS F, SUI Y, GUAN T, MILLER N, TAN Y, JIN W, CARRANO AC, HUISING MO, SHIRIHAI OS, YEO GW, SANDER M. 2017 Pseudotemporal ordering of single cells reveals metabolic control of postnatal beta cell proliferation. Cell Metabolism 25(5):1160-1175.

49. VAN DER MEULEN T, MAWLA AM, DIGRUCCIO MR, ADAMS MW, NIES V, DÓLLEMAN S, LIU S, ACKERMANN AM, CÁCERES E, HUNTER AU, KAESTNER KH, DONALDSON CJ, HUISING MO. 2017 Virgin Beta Cells Persist throughout Life at a Neogenic Niche within Pancreatic Islets. Cell Metabolism 4:911-926. Selected as ‘Best of Cell Metabolism 2017.’

50. VAN DER MEULEN T, LEE S, NOORDELOOS E, DONALDSON CJ, ADAMS MW, NOGUCHI GM, MAWLA AM, HUISING MO. 2018 Artemether Does Not Turn Alpha Cells into Beta Cells. Cell Metabolism 9:218-225.

51. RORSMAN P, HUISING MO. 2018 The somatostatin-secreting pancreatic d-cell in health and disease. Nature Reviews in Endocrinology, 14(7):404-414.

52. VILARINO M, SUCHY FP, RASHID ST, LINDSAY H, REYES J, MCNABB BR, VAN DER MEULEN T, HUISING MO, NAKAUCHI H, ROSS PJ. 2018 Mosaicism diminishes the value of pre-implantation embryo biopsies for detecting CRISPR/Cas9 induced mutations in sheep. Transgenic Research 27(6):525-537.

53. HUISING MO, LEE S, VAN DER MEULEN T. 2018 Evidence for a Neogenic Niche at the Periphery of Pancreatic Islets. BioEssays 40(11). Topic of an Editorial in: BioEssays 40(11).

54. HUISING MO, VAN DER MEULEN T, HUANG JL, POURHOSSEINZADEH MS, NOGUCHI GM. 2018 The Difference d-cells Make in Glucose Control. Physiology 33(6):403-411. Topic of an Editorial in: Physiology (Sieck, 33(6):372-373).

55. MAWLA AM, HUISING MO. 2019 Navigating the Depths and Avoiding the Shallows of Pancreatic Islet Cell Transcriptomes. Diabetes, 68(7):1380-1393.

56. NOGUCHI GM, HUISING MO. 2020 Integrating the Inputs that Shape Pancreatic Islet Hormone Release. Nature Metabolism 1 (12), 1189-1201.

57. PIRES J, GREATHOUSE RL, QUACH N, HUISING MO, CRAKES KR, MILLER M, GILOR C. 2020 The effect of the ghrelin-receptor agonist capromorelin on glucose metabolism in healthy cats. Domestic Animal Endocrinology 74:106484.

58. HUANG JL, LEE S, HOEK P, VAN DER MEULEN T, VAN R, HUISING MO. 2020 Genetic deletion of Urocortin 3 does not prevent functional maturation of beta cells. Journal of Endocrinology, 246(1):69-78.

59. HUISING MO. 2020 Paracrine regulation of insulin secretion. Diabetologia 63(10):2057- 2063.

60. CROZE ML, FLISHER ML, GUILLAUME A, TREMBLAY C, CAMPBELL SA, NOGUCHI GM, CASTILLO VC, GRANZIERA S, VIVOT K, GHISLAIN J, HUISING MO, POITOUT V. 2021 Free fatty acid receptor 4 inhibitory signaling in delta cells regulates islet hormone secretion in mice. Molecular Metabolism, 45.

61. LEE S, ZHANG J, SARAVANAKUMAR S, FLISHER MF, GRIMM DG, VAN DER MEULEN T, HUISING MO. 2021 Virgin beta cells at the neogenic niche proliferate normally and mature slowly. Diabetes 70(5):1070-1083.

62. KOIKE S, HSU MF, BETTAIEB A, CHU N, MATSUMOTO N, MORISSEAU C, HAVEL PJ, HUISING MO, HAMMOCK BD, HAJ, FG. 2021 Genetic deficiency or pharmacological inhibition of soluble epoxide hydrolase ameliorates high fat diet-induced pancreatic beta cell dysfunction and loss. Free Radical Biology and Medicine 172:48-57.

63. BLENCOWE M, FURTERER A, WANG Q, GAO F, ROSENBERGER M, PEI L, NOMOTO H, MAWLA AM, HUISING MO, COPPOLA G, YANG X, BUTLER PC, GURLO T. 2022 IAPP-induced beta cell stress recapitulates the islet transcriptome in type 2 diabetes. Diabetologia 65(1):173-187.

64. FLISHER MF, SHIN D, HUISING MO. 2022 Urocortin3: local inducer of somatostatin release and bellwether of beta cell maturity. Peptides, in press.

Book Chapters

(1) DIGRUCCIO MR and HUISING MO. 2016 Corticotropin Releasing Hormone (CRH) and Urocortins are Key Central and Peripheral Regulators of Stress, Anxiety, Energy Balance and Metabolism. Neuroscience and Biobehavioral Psychology, Elsevier.

(2) VISSERS M, GORISSEN M, FLIK G, HUISING MO. 2010 Leptin in teleost fish. In: Paolucci M (ed.), Leptin in Non-mammalian Vertebrates, pp. 1-24. Transworld Research Network.

(3) HUISING MO, VALE WW. 2008 CRF and urocortins: binding proteins and receptors. In: Squire LR (ed.), Encyclopedia of Neuroscience, volume 3, pp. 231-237. Oxford: Ac. Press.